CRISPR in Tomato
CRISPR in Tomato
Embedded Files
Alex Maioli, Federica De Marchi, Danila Valentino, Silvia Gianoglio, Davide Patono, Fabio Miloro, Yuling Bai, Cinzia Comino, Sergio Lanteri, Claudio Lovisolo, Alberto Acquadro, Andrea Moglia (2024) Knock-out of SlDMR6-1 in tomato promotes a drought-avoidance strategy and increases tolerance to Late Blight, Plant Stress, https://doi.org/10.1016/j.stress.2024.100541
Maioli Alex, De Marchi Federica, Valentino Danila, Gianoglio Silvia, Patono Davide, Miloro Fabio, Bai Yuling, Comino Cinzia, Lanteri Sergio, Lovisolo Claudio, Acquadro Alberto, Moglia Andrea (2023) New insights on the role of SlDMR6-1 in drought avoidance in tomato. bioRxiv 12.14.571645; doi: https://doi.org/10.1101/2023.12.14.571645
Ruiling Li, Lei Cui, Matteo Martina, Valentina Bracuto, Fien Meijer-Dekens, Anne-Marie A. Wolters, Andrea Moglia, Yuling Bai, Alberto Acquadro (2024) Less is More: CRISPR/Cas9-based Mutations in DND1 Gene Enhance Tomato Resistance to Powdery Mildew with Low Fitness Costs, BMC Plant Biology; https://doi.org/10.1186/s12870-024-05428-3, accepted paper
Ruiling Li, Alex Maioli, Sergio Lanteri, Andrea Moglia, Yuling Bai, Alberto Acquadro (2023) Genomic Analysis Highlights Putative Defective Susceptibility Genes in Tomato Germplasm. Plants (Basel). Jun 12;12(12):2289. PMID: 37375913; PMCID: PMC10300993. https://doi.org/10.3390/plants12122289
Ruiling Li, Alex Maioli, Yan Zhe, Yuling Bai, Danilo Valentino, Anna Maria Milani, Valerio Pompili, Cinzia Comino, Sergio Lanteri, Andrea Moglia, Alberto Acquadro (2022) CRISPR/Cas9-based knock-out of the PMR4 gene reduces susceptibility to late blight in two tomato cultivars. International Journal of Molecular Sciences - https://doi.org/10.3390/ijms232314542
Gianoglio S, Comino C, Moglia A, Acquadro A, García-Carpintero V, Diretto G, Sevi F, Rambla JL, Dono G, Valentino D, Moreno-Giménez E, Fullana-Pericàs M, Conesa MÀ, Galmès L, Lanteri S, Mazzucato A, Orzáez D and Granell A (2022) In-depth characterization of greenflesh tomato mutants obtained by CRISPR/Cas9 editing: a case study with implications for breeding and regulation. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2022.936089
Editing of Susceptibility Genes (S-genes)
Editing of Susceptibility Genes (S-genes)
Late blight (LB), caused by Phytophthora infestans, and powdery mildew (PM), caused by species within the Erysiphaceae family, are the most destructive and widespread diseases in tomato (Solanum lycopersicum L.). The control of these pathogens extensively relies on fungicide application, leading to economic losses and environment impact.
The disabling of susceptibility (S) genes, which facilitate pathogen infection, may provide a broad-spectrum and durable type of resistance. To this purpose, the CRISPR/Cas9 gene editing represents the most suitable and fast strategy.
We selected the Powdery Mildew Resistant 4 (PMR4) and Downy Mildew Resistant 6 (PMR4) genes, whose disabling has been shown previously observd to confer PM/DM resistance in other species.
Our results will provide suitable tools for biotechnological applications and open the way to the development of pathogen resistant tomato genotypes.
In vitro tomato plantlets
Ruiling Li, Alex Maioli, Yan Zhe, Yuling Bai, Anna Maria Milani, Danilo Valentino, Valerio Pompili, Cinzia Comino, Sergio Lanteri, Andrea Moglia, Alberto Acquadro. Gene editing of PMR4 promotes resistance in tomato varieties towards late blight (LB) attacks (2021) SIGA congress, Session 4, https://sigaannualcongress.it - Poster Award
A. Maioli, F. Miloro, S. Gianoglio, R. Li, D. Valentino, A.M. Milani, C. Comino, S. Lanteri, A. Acquadro, A. Moglia. CRISPR/Cas9-mediated Knock-out of tomato DMR6 gene to confer resistance against pathogens (2021) SIGA congress, Session 4, https://sigaannualcongress.it
Page updated
Report abuse